5/21/2023 0 Comments Moso bamboo seedsCalli were induced on MS medium added 4–6 mg⋅L –1 2,4-dichlorophenoxyacetic acid (2,4-D) with high efficiency (>60%). Here, we established a plant regeneration system from immature embryos. Without a genetic transformation system, it is difficult to verify the functions of genes controlling important traits and conduct molecular breeding in moso bamboo. Moso bamboo ( Phyllostachys edulis ) is the most important monopodial bamboo species worldwide. The present work showed the feasibility of using bamboo spikelets as explants and the efficiency of using 2-iP in combination with 2,4-D to callus induction and in vitro plant regeneration through somatic embryogenesis. Somatic embryos transferred to a plant growth regulator-free medium resulted in plantlet germination. The subsequent reduction of this auxin to 4.5 µM resulted in somatic embryo maturation. Additionally, the effect of explant cutting in half and its orientation on the culture medium were tested to improve callus induction The 2,4-D was essential for callus induction, and in combination with the two cytokinins resulted in embryogenic callus induction and somatic embryos regeneration. The different calli types that developed were characterized and subcultured in 0, 4.5, 9, and 18 µM of 2,4-D in combination with 9 µM of 2-Isopentenyladenine (2-iP) or Kinetin for multiplication and somatic embryos regeneration. Pre-anthesis spikelets were collected, disinfected, and subjected to callus induction on MS basal medium supplemented by 2,4-Dichlorophenoxyacetic acid (2,4-D) at 0, 9, 18, 27, and 36 µM in combination with 9 µM of 2-iP or 9 µM Kinetin. The present work aimed to establish in vitro callus culture and plant regeneration through somatic embryogenesis starting from young spikelets of D. Besides its use as edible sweet shoots and culms for structural uses, the giant bamboo, Dendrocalamus asper is an imposing ornamental bamboo for horticulture. This review will provide researchers with a comprehensive overview and better understanding of bamboo biotechnology for better utilization and adaptation as a sustainable bioresource.īamboos are an important worldwide non-timber forest product with rising interest due to their environmentally friendly applications. In addition, the application of next-generation sequencing technology to bamboo plants will be examined for fundamental processes related to growth and stress regulation, lignin and cellulose biosynthesis, secondary cell wall deposition, and flowering behavior. This review discusses protocols for in vitro propagation, in vitro flowering, synthetic seed production, and transgenic techniques used to improve bamboo. Genetic transformation of bamboo species remains limited, although the bamboo genome is now better understood and genetic improvement is possible. ![]() ![]() ![]() Seed propagation is ineffective for bamboo due to low seed viability, while vegetative propagation is limited to small-scale production. Biotechnology applications, such as in vitro propagation and tissue culture, offer a range of solutions for improved and controlled growth, resulting in a more effective and reliable production chain. Sustainable production is needed to combat industrial overexploitation by the paper, pulp, and fuel industries, as well as improper resource management that has widened the gap between supply and demand. Bamboo, a fast growing plant, is a valuable and renewable bioresource with great socioeconomic value.
0 Comments
Leave a Reply. |